Throughout July, the Oxford Bus Company is donating 50p from every return ticket purchased on the X90 to Helen & Douglas House.
All proceeds raised from return journey sales between Oxford and London in July will be donated directly to the Oxfordshire-based hospice.
After ten days, the campaign already raised £1,500.
Helen & Douglas House provides hospice care for babies and children in Oxfordshire and surrounding countries.
Oxford Bus Company, Managing Director, Phil Southall, says: “When possible, we like to give something back to the community we are proud to serve and share.”