The Oxford Bus Company has donated £5,070 to Helen & Douglas House, raised from 50p pledges from every return ticket purchased on the X90 during July.
Staff from the operator were also treated to a guided tour of the centre to see first-hand the vital services provided.
Oxford Bus Company MD, Phil Southall, says: “It was a pleasure to have a tour of the Helen & Douglas House and meet the staff who do such a fantastic job.
Helen & Douglas House provides hospice care for babies and children in Oxfordshire and surrounding counties.
